Actb^tm1Npa
Targeted Allele Detail

Summary

• Symbol: Actb^tm1Npa
• Name: actin, beta; targeted mutation 1, Novartis Pharma AG
• MGI ID: MGI:3770046
• Synonyms: beta-actin EGFP, TTG4
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^tm1Npa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:129639
• Parent Cell Line: BALB/c-I (ES Cell)
• Strain of Origin: BALB/cJ

Mutation description

• Allele Type: Targeted (RMCE-ready, Reporter)
• Mutation: Insertion
• Mutation details: A recombinase-mediated cassette exchange (RMCE) vector consisting of a mutant LoxP site, a promoterless EGFP reporter gene, a neomycin selection cassette and a wt LoxP site was inserted into the ATG translational start site of exon 2. Endogenous gene expression was replaced with EGFP expression as assessed by fluorescent microscopy and immunohistochemistry of brain, heart, liver and other organs. This allele allows for RMCE into the b-actin locus. (J:129639)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 53 strains or lines available

References

• Original: J:129639 Shmerling D, et al., Strong and ubiquitous expression of transgenes targeted into the beta-actin locus by Cre/lox cassette replacement. Genesis. 2005 Aug;42(4):229-35
• All: 7 reference(s)