Tg(Ins2-Chrm3*)1Jwe
Transgene Detail

Summary

• Symbol: Tg(Ins2-Chrm3*)1Jwe
• Name: transgene insertion 1, Jurgen Wess
• MGI ID: MGI:3770248
• Synonyms: beta-M3-Tg1
• Transgene: Tg(Ins2-Chrm3*)1Jwe Location: unknown
• Alliance: Tg(Ins2-Chrm3*)1Jwe page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-Chrm3*)1Jwe expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Chrm3 (MGI:88398)

• Tg(Ins2-Chrm3*)1Jwe expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The rat insulin 2 promoter was used to drive expression of the gene in beta cells. Chrm3 contains two modifications (an N-terminal 9 amino acid hemagglutinin epitope tag was added and a central portion of the third intracellular loop, Ala274 to Lys469, was deleted) that do not change its functionality but allow the researchers to distinguish it from the endogenous gene product. (J:129643)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two lines were created (1 and 2) with identical phenotypes.

References

• Original: J:129643 Gautam D, et al., A critical role for beta cell M3 muscarinic acetylcholine receptors in regulating insulin release and blood glucose homeostasis in vivo. Cell Metab. 2006 Jun;3(6):449-61
• All: 2 reference(s)