Tln1^tm4Crit
Targeted Allele Detail

Summary

• Symbol: Tln1^tm4Crit
• Name: talin 1; targeted mutation 4, David R Critchley
• MGI ID: MGI:3770448
• Synonyms: targeted Neo allele, Tln1^flN, Tln1(flNeo)
• Gene: Tln1 Location: Chr4:43531513-43562583 bp, - strand Genetic Position: Chr4, 23.05 cM
• Alliance: Tln1^tm4Crit page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131145
• Parent Cell Line: E14.1a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP-flanked neomycin resistance cassette (Neo) is present in intron 1, upstream of the first coding exon (exon 2), and a third loxP site resides downstream of the fourth coding exon (exon 5). Partial Cre-mediated recombination in vivo was used to delete only the floxed Neo cassette and create the Tln1^tm4.1Crit allele. (J:131145)
• Inheritance: Recessive

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tln1 Mutation: 147 strains or lines available

Notes

Cre recombinase-mediated deletion of the Neo cassette from the present, lethal mutation generates a floxed allele for which homozygosity has no adverse effect on survival or fertility.

References

• Original: J:131145 Monkley SJ, et al., Creation of a conditional knock-out allele of Tln1. MGI Direct Data Submission. 2008
• All: 1 reference(s)