Tead4^tm1Hssk
Targeted Allele Detail

Summary

• Symbol: Tead4^tm1Hssk
• Name: TEA domain family member 4; targeted mutation 1, Hiroshi Sasaki
• MGI ID: MGI:3770620
• Gene: Tead4 Location: Chr6:128204106-128277776 bp, - strand Genetic Position: Chr6, 62.92 cM
• Alliance: Tead4^tm1Hssk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130047
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by insertion into exon 2, which encodes the DNA-binding TEA domain of the protein, of two functional cassettes: 1) a loxP-flanked Pgk-neo-poly(A) cassette, followed by 2) a cDNA encoding the beta-geo (E. coli beta-galactosidase/neomycin resistance) fusion protein flanked 5' by an internal ribosomal entry site (IRES) and 3' by a polyadenylation signal. Reverse transcription- (RT-) PCR using primers in exons 5 and 6 detects no transcripts from the mutant allele in homozygous E3.5 embryos. Whereas immunohistochemical analysis with antisera to TEAD4 stains all cell nuclei of 34-36-cell wild-type embryos, mutant embryos produce no signal. (J:130047)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:	36 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tead4 Mutation: 75 strains or lines available

References

• Original: J:130047 Nishioka N, et al., Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech Dev. 2008 Mar-Apr;125(3-4):270-83
• All: 5 reference(s)