Fgg^tm1Lord
Targeted Allele Detail

Summary

• Symbol: Fgg^tm1Lord
• Name: fibrinogen gamma chain; targeted mutation 1, Susan T Lord
• MGI ID: MGI:3771182
• Synonyms: V/F IV mouse
• Gene: Fgg Location: Chr3:82915031-82922356 bp, + strand Genetic Position: Chr3, 36.94 cM
• Alliance: Fgg^tm1Lord page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130183
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A targeting vector was constructed with an in-frame deletion of the six bp within exon 8 that encode for asparagine 319 and aspartic acid 320. This mutation was predicted to prevent calcium binding and protein polymerization. For diagnostic purposes, a Xho I restriction site was removed and a Sca I site was added within exon 8, and a Bgl II site was inserted 68 bp upstream from the start of exon 8. The vector replaced exon 8 and inserted a pgk-HPRT selection cassette into intron 10. Protein isolated from heterozygous mice was not fully protected from plasmin cleavage in the presence of calcium demonstrating altered calcium binding by the mutated protein. (J:130183)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgg Mutation: 30 strains or lines available

References

• Original: J:130183 Hogan KA, et al., Synthesis of a mouse model of the dysfibrinogen Vlissingen/Frankfurt IV. Ann N Y Acad Sci. 2001;936:117-21
• All: 2 reference(s)