Hpgd^tm1Ljm
Targeted Allele Detail

Summary

• Symbol: Hpgd^tm1Ljm
• Name: hydroxyprostaglandin dehydrogenase 15 (NAD); targeted mutation 1, Louis J Muglia
• MGI ID: MGI:3771208
• Synonyms: Hpgd^H
• Gene: Hpgd Location: Chr8:56747620-56774078 bp, + strand Genetic Position: Chr8, 29.7 cM, cytoband B3.2
• Alliance: Hpgd^tm1Ljm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130001
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: In the process of creating a loxP-flanked allele, a tandem duplication of exon 1 and 2 with an frt-flanked neo cassete and a 5' loxP site inserted into intron 2 was knocked into the gene. The authors state that this allele is hypomorphic. Northern blot analysis confirmed reduced transcript levels in the lungs and the absence of transcript in the liver and placenta of E17.5 embryos. Enzymatic assays confirmed decreased activity in the adult lungs, liver and E15.5 placenta. (J:130001)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hpgd Mutation: 24 strains or lines available

References

• Original: J:130001 Roizen JD, et al., Preterm Birth without Progesterone Withdrawal in 15-Hydroxyprostaglandin Dehydrogenase Hypomorphic Mice. Mol Endocrinol. 2008 Jan;22(1):105-12
• All: 1 reference(s)