B4galt1^tm2Shur
Targeted Allele Detail

Summary

• Symbol: B4galt1^tm2Shur
• Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1; targeted mutation 2, Barry D Shur
• MGI ID: MGI:3771315
• Synonyms: GalT-null, Long GalT-Null, long gt^-
• Gene: B4galt1 Location: Chr4:40804602-40854005 bp, - strand Genetic Position: Chr4, 20.46 cM
• Alliance: B4galt1^tm2Shur page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43773
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to insert a point mutation into the first translation initiation codon in exon 1, which initiates translation of the long isoform. Expression of the long isoform is eliminated. Expression of the short isoform is unaffected. (J:43773)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any B4galt1 Mutation: 43 strains or lines available

References

• Original: J:43773 Lu Q, et al., Sperm from beta 1,4-galactosyltransferase-null mice are refractory to ZP3-induced acrosome reactions and penetrate the zona pellucida poorly. Development. 1997 Oct;124(20):4121-31
• All: 3 reference(s)