Pou5f1^tm1Jae
Targeted Allele Detail

Summary

• Symbol: Pou5f1^tm1Jae
• Name: POU domain, class 5, transcription factor 1; targeted mutation 1, Rudolf Jaenisch
• MGI ID: MGI:3771368
• Synonyms: Oct4-IRES-GFPneo, Oct4-neo
• Gene: Pou5f1 Location: Chr17:35816929-35821674 bp, + strand Genetic Position: Chr17, 18.69 cM
• Alliance: Pou5f1^tm1Jae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130250
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The Oct4-IRES-GFPneo targeting vector was designed to insert an IRES-GFPneo fusion cassette downstream of exon 5. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. (J:130250)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pou5f1 Mutation: 82 strains or lines available

Notes

When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts.

References

• Original: J:130250 Wernig M, et al., In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature. 2007 Jul 19;448(7151):318-24
• All: 4 reference(s)