Phb1^{Gt(XT0035)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Phb1^{Gt(XT0035)Wtsi}
• Name: prohibitin 1; gene trap XT0035, Wellcome Trust Sanger Institute
• MGI ID: MGI:3771851
• Synonyms: PHB^-
• Gene: Phb1 Location: Chr11:95557783-95571599 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Phb1^{Gt(XT0035)Wtsi} page

Mutation origin

• Mutant Cell Line: XT0035
• Germline Transmission: Earliest citation of germline transmission: J:130064
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxf
• Mutation details: The gene trap containing the En2 intron 1, a splice acceptor and a beta-geo cassette with a poly adenylation signal was inserted into exon 7. Decreased protein expression was confirmed in heterozygote epithelial cells by western blot analysis. (J:130064)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XT0035	CW991616 ; (MGI Seq Detail)	5' RACE	multiple
  * GRCm39; (strand of gene trap mutagenesis)

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phb1 Mutation: 25 strains or lines available

References

• Original: J:130064 He B, et al., A repressive role for prohibitin in estrogen signaling. Mol Endocrinol. 2008 Feb;22(2):344-60
• All: 2 reference(s)