Dmbt1^tm1Janm
Targeted Allele Detail

Summary

• Symbol: Dmbt1^tm1Janm
• Name: deleted in malignant brain tumors 1; targeted mutation 1, Jan Mollenhauer
• MGI ID: MGI:3771872
• Synonyms: Dmbt1^-
• Gene: Dmbt1 Location: Chr7:130633787-130723357 bp, + strand Genetic Position: Chr7, 73.2 cM, cytoband F4
• Alliance: Dmbt1^tm1Janm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130258
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted about 1kb upstream of exon 1 and a loxP-flanked neo selection cassette was inserted into intron 1. Transient transfection of Cre-recombinase deleted the genomic sequence encoding part of the promoter, the upstream untranslated region of the gene, exon 1 which contains the ATG site and part of the coding sequence for the signal peptide, and a portion of intron 1. A single loxP site remained in place of the deleted gene sequence. Gene inactivation was confirmed by Northern and Western analysis of small intestine tissue. (J:130258)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dmbt1 Mutation: 111 strains or lines available

References

• Original: J:130258 Renner M, et al., DMBT1 confers mucosal protection in vivo and a deletion variant is associated with Crohn's disease. Gastroenterology. 2007 Nov;133(5):1499-509
• All: 5 reference(s)