Pou5f1^tm2Jae
Targeted Allele Detail

Summary

• Symbol: Pou5f1^tm2Jae
• Name: POU domain, class 5, transcription factor 1; targeted mutation 2, Rudolf Jaenisch
• MGI ID: MGI:3772191
• Synonyms: Oct4-EGFP, Oct4-GFP, Oct4-iresGFP, Oct4-IRES-GFP, Pou5f1^tm2(EGFP)Jae
• Gene: Pou5f1 Location: Chr17:35816929-35821674 bp, + strand Genetic Position: Chr17, 18.69 cM
• Alliance: Pou5f1^tm2Jae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130248
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert an "IRES-EGFP-floxed NEO" cassette (containing an internal ribosomal entry site (IRES), enhanced green fluorescent protein (EGFP) sequence, and a loxP-flanked neo) between the stop codon and endogenous polyA signal of the targeted gene. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the neo selection cassette. (J:130248)

Expression

In Mice Carrying this Mutation:

4 assay results 5 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pou5f1 Mutation: 82 strains or lines available

Notes

When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-EGFP murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts.

References

• Original: J:130248 Lengner CJ, et al., Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell. 2007 Oct 11;1(4):403-415
• All: 37 reference(s)