Polr2a^{tm1(cre/ERT2)Bbd}
Targeted Allele Detail

Summary

• Symbol: Polr2a^{tm1(cre/ERT2)Bbd}
• Name: polymerase (RNA) II (DNA directed) polypeptide A; targeted mutation 1, Mariano Barbacid
• MGI ID: MGI:3772332
• Synonyms: Polr2a^{tm1(cre/ESR1)Bbd}, RERT, RERTert, RERTn, RERTn^ert, RNAPII(CreERT2)
• Gene: Polr2a Location: Chr11:69624823-69649459 bp, - strand Genetic Position: Chr11, 42.86 cM, cytoband B1-C
• Alliance: Polr2a^{tm1(cre/ERT2)Bbd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86101
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Polr2a^{tm1(cre/ERT2)Bbd} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Polr2a (MGI:98086)

• Mutation details: An IRES-cre/ERT2 cassette was knocked in between the termination codon and the polyadenylation signal to allow for biscistronic inducible expression of cre. (J:98944)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Polr2a (mouse) Summary of all recombinase alleles driven by Polr2a. Alliance mouse Polr2a gene page

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Polr2a^{tm1(cre/ERT2)Bbd}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Polr2a Mutation: 92 strains or lines available

References

• Original: J:86101 Guerra C, et al., Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context. Cancer Cell. 2003 Aug;4(2):111-20
• All: 51 reference(s)