Sgca^tm2Kcam
Targeted Allele Detail

Summary

• Symbol: Sgca^tm2Kcam
• Name: sarcoglycan, alpha (dystrophin-associated glycoprotein); targeted mutation 2, Kevin P Campbell
• MGI ID: MGI:3772334
• Gene: Sgca Location: Chr11:94853617-94867153 bp, - strand Genetic Position: Chr11, 59.01 cM, cytoband C
• Alliance: Sgca^tm2Kcam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130252
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector was designed to introduce an H77C (TGC->CAC) missense substitution in exon 3, and place a loxP-flanked pgk-neomycin resistance cassette (in opposite transcriptional orientation) into intron 3 of the gene. Alpha-sarcoglycan mRNA is not detected by RT-PCR, and protein is not detected by Western blot or immunofluorescence staining of skeletal muscle. (J:130252)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sgca Mutation: 26 strains or lines available

References

• Original: J:130252 Kobuke K, et al., A common disease-associated missense mutation in alpha-sarcoglycan fails to cause muscular dystrophy in mice. Hum Mol Genet. 2008 May 1;17(9):1201-13
• All: 3 reference(s)