Tg(CAG-SAC/EGFP)35Rang
Transgene Detail

Summary

• Symbol: Tg(CAG-SAC/EGFP)35Rang
• Name: transgene insertion 35, Vivek Rangnekar
• MGI ID: MGI:3772442
• Synonyms: SAC/35, SAC-GFP line 35, Tg(ACTB-SAC/EGFP)35Rang
• Transgene: Tg(CAG-SAC/EGFP)35Rang Location: unknown
• Alliance: Tg(CAG-SAC/EGFP)35Rang page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The pCA/SAC-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) enhancer) upstream of a fusion gene containing a Par-4 SAC domain (Pawr gene, amino acids 137-195) cDNA fused to an enhanced green fluorescent protein (EGFP), all followed by a polyA sequence. The resulting transgenic offspring were bred to (C57BL/6 x C3H)F1 mice. Founder line 35 mice (SAC/35; found to have 6-8 copies of the transgene). Widespread expression of the transgene is observed in all tested tissues (with some differential tissue-specific regulation of transgene expression or protein stability reported). EGFP expression level is appropriate for immunoblots, but not sufficient enough for fluorescence of flow cytometry applications. (J:125633)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:125633 Zhao Y, et al., Cancer resistance in transgenic mice expressing the SAC module of Par-4. Cancer Res. 2007 Oct 1;67(19):9276-85
• All: 1 reference(s)