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Gt(ROSA)26Sortm1(HBEGF)Awai
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm1(HBEGF)Awai
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Ari Waisman
MGI ID: MGI:3772576
Synonyms: Gt(ROSA)26Sortm1(Dtr)ThBu, iDTR, R26iDTR, ROSA26-DTR, Rosa26iDTR, Rosa26-LSL-DTR, Rosa-iDTR, RosaiDTR
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm1(HBEGF)Awai page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:131076
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence)
Mutation:    Insertion
 
Gt(ROSA)26Sortm1(HBEGF)Awai expresses 1 gene
 
Mutation details: A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. (J:131076)
Phenotypes
Key:
hm homozygous ht heterozygous tg involves transgenes √ phenotype observed
cn conditional genotype  cx complex: > 1 genome feature ot other: hemizygous, indeterminate,... N normal phenotype
Genotype/
Background:
Allelic Composition
Genetic Background
Cell Line(s)
Allelic CompositionGenetic BackgroundCell Line(s)
cn1
Cd19tm1(cre)Cgn/?
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai
involves: 129P2/OlaHsd * C57BL/6
 
cn2
ApcMin/Apc+
Dclk1tm1.1(cre/ERT2)Seno/Dclk1+
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sortm1(HBEGF)Awai
involves: 129S4/SvJaeSor * C57BL/6
 
cn3
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm9(CAG-tdTomato)Hze
Oxttm1.1(cre)Dolsn/Oxt+
involves: 129S6/SvEvTac * C57BL/6
 
cn4
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+
Kiss1rtm1.1(cre)Uboe/Kiss1r+
involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * SJL
 
cn5
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+
Kiss1tm1.1(cre)Uboe/Kiss1+
involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * SJL
 
cn6
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+
Opn4tm1(cre)Sapa/Opn4+
involves: 129S * C57BL/6
 
cn7
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai
Tg(Gdf9-icre)5092Coo/0
involves: C57BL/6
 
cn8
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai
Mogtm1(cre)Gkl/?
involves: C57BL/6
 
cn9
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+
Tg(Mrgpra3-GFP/cre)#Xzd/0
involves: C57BL/6
 
cn10
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sortm1(HBEGF)Awai
Tg(Cd4-cre)1Cwi/0
involves: C57BL/6 * DBA/2
 
cn11  Disease Model
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+
Tg(Gh1-cre)bKnmn/0
involves: C57BL/6 * FVB/N
 
cn12
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+
Tg(Krt1-15-cre/PGR*)22Cot/0
involves: C57BL/6J * SJL/J
 
cn13
Gt(ROSA)26Sortm1(HBEGF)Awai/Gt(ROSA)26Sor+
Tg(Adora2a-cre)2MDkde/0
involves: C57BL/6 * SJL
 
Phenotypes:
Affected Systems
cn1
cn2
cn3
cn4
cn5
cn6
cn7
cn8
cn9
cn10
cn11
cn12
cn13
show or hide all annotated terms Sex:
                     
adipose tissue
√ √ √
increased subcutaneous adipose tissue amount
√
increased total body fat amount
√ √ √
increased retroperitoneal fat pad weight
√
behavior/neurological
N N √ √ N √ √
behavior/neurological phenotype
N N N N
enhanced conditioned place preference behavior
√
decreased fluid intake
√
impaired pupillary reflex
√
tremors
√
hyperactivity
√
hindlimb paralysis
√
abnormal circardian behavior entrainment
√
cellular
√ √ √
abnormal female germ cell apoptosis
√
increased neuron apoptosis
√
abnormal granulosa cell apoptosis
√
increased cell proliferation
√
digestive/alimentary system
N √
digestive/alimentary phenotype
N
abnormal esophageal epithelium morphology
√
endocrine/exocrine glands
N √ √
endocrine/exocrine gland phenotype
N
abnormal granulosa cell apoptosis
√
decreased somatotroph cell number
√
small adenohypophysis
√
abnormal ovary morphology
√
abnormal ovarian follicle morphology
√
growth/size/body
N √ √ √
growth/size/body region phenotype
N N
decreased lean body mass
√
decreased body weight
√
weight loss
√
increased susceptibility to diet-induced obesity
√
hematopoietic system
√ √
abnormal bone marrow cell number
√
abnormal lymphocyte morphology
√
abnormal lymphocyte cell number
√ √
absent immature B cells
√
absent mature B cells
√
decreased single-positive T cell number
√
abnormal B cell morphology
√
abnormal splenic cell ratio
√
homeostasis/metabolism
√ √ √
abnormal circulating hormone level
√
decreased circulating insulin level
√
decreased circulating insulin-like growth factor I level
√
increased circulating leptin level
√
decreased circulating growth hormone level
√
abnormal energy expenditure
√
decreased energy expenditure
√
increased susceptibility to diet-induced obesity
√
abnormal gas homeostasis
√
decreased oxygen consumption
√ √ √
increased respiratory quotient
√
abnormal glucose homeostasis
√
decreased circulating glucose level
√
impaired glucose tolerance
√
increased insulin sensitivity
√
decreased liver triglyceride level
√
decreased response to leptin
√ √
immune system
√ √ √
abnormal lymphocyte morphology
√
abnormal lymphocyte cell number
√ √
absent immature B cells
√
absent mature B cells
√
decreased single-positive T cell number
√
abnormal B cell morphology
√
abnormal splenic cell ratio
√
abnormal lymph node cell ratio
√
abnormal immune system physiology
√
integument
√ √
increased subcutaneous adipose tissue amount
√
decreased pruritus
√
liver/biliary system
√
decreased liver triglyceride level
√
decreased liver weight
√
neoplasm
√
abnormal tumor morphology
√
nervous system
√ √ √ √ √ √ √ √
nervous system phenotype
N
increased neuron apoptosis
√
decreased somatotroph cell number
√
small adenohypophysis
√
abnormal basal ganglion morphology
√
abnormal suprachiasmatic nucleus morphology
√
astrocytosis
√
abnormal neuron morphology
√
decreased neuron number
√ √ √
decreased sensory neuron number
√
decreased retina ganglion cell number
√
demyelination
√
reproductive system
N √ √
reproductive system phenotype
N
abnormal female germ cell apoptosis
√
abnormal granulosa cell apoptosis
√
abnormal ovary morphology
√
abnormal ovarian follicle morphology
√
abnormal estrous cycle
√
prolonged diestrus
√
prolonged estrus
√
female infertility
√
vision/eye
√
impaired pupillary reflex
√
decreased retina ganglion cell number
√
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Key:
√ disease model   expected model not found
Models:
Human Diseases
cn11
IDs
 √
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  1024 strains or lines available
References
Original:  J:131076 Buch T, et al., A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods. 2005 Jun;2(6):419-26
All:  475 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory