Gt(ROSA)26Sor^{tm1(HBEGF)Awai}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(HBEGF)Awai}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Ari Waisman
• MGI ID: MGI:3772576
• Synonyms: Gt(ROSA)26Sor^tm1(Dtr)ThBu, iDTR, R26^iDTR, ROSA26-DTR, Rosa26iDTR, Rosa26-LSL-DTR, Rosa-iDTR, RosaiDTR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(HBEGF)Awai} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131076
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(HBEGF)Awai} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  monkey	HBEGF

• Mutation details: A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. (J:131076)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:131076 Buch T, et al., A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods. 2005 Jun;2(6):419-26
• All: 455 reference(s)