Kcnq1ot1^tm2Mjh
Targeted Allele Detail

Summary

• Symbol: Kcnq1ot1^tm2Mjh
• Name: KCNQ1 overlapping transcript 1; targeted mutation 2, Michael J Higgins
• MGI ID: MGI:3772666
• Synonyms: pA, YJ69
• Gene: Kcnq1ot1 Location: Chr7:142766848-142850284 bp, - strand Genetic Position: Chr7, 88.15 cM
• Alliance: Kcnq1ot1^tm2Mjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130529
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A polyadenylation signal in the correct orientation followed by a floxed neo cassette was inserted downstream of the endogenous promoter. The neo cassette was removed by cre-mediated recombination. The expression of RNA upstream of the insertion and the absence of expression of RNA downstream of the insertion was confirmed by RNase protection assay and RT-PCR. (J:130529)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnq1ot1 Mutation: 0 strains or lines available

References

• Original: J:130529 Shin JY, et al., Two distinct mechanisms of silencing by the KvDMR1 imprinting control region. EMBO J. 2008 Jan 9;27(1):168-78
• All: 2 reference(s)