Nampt^{Gt(RRT307)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Nampt^{Gt(RRT307)Byg}
• Name: nicotinamide phosphoribosyltransferase; gene trap RRT307, BayGenomics
• MGI ID: MGI:3772777
• Synonyms: Nampt^-
• Gene: Nampt Location: Chr12:32870334-32903368 bp, + strand Genetic Position: Chr12, 13.74 cM, cytoband B1
• Alliance: Nampt^{Gt(RRT307)Byg} page

Mutation origin

• Mutant Cell Line: RRT307
• Germline Transmission: Earliest citation of germline transmission: J:130534
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The beta-geo gene trap was inserted between exon 8 and 9. The truncated fusion protein produced lacks the critical residues necessary for enzymatic activity. Authors state that this is an enzymatically null allele. (J:130534)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRT307	CL315248 ; (MGI Seq Detail)	5' RACE	Chr12:32890982-32892800 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  49 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nampt Mutation: 93 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:130534 Revollo JR, et al., Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme. Cell Metab. 2007 Nov;6(5):363-75
• All: 3 reference(s)