Tsix/Xist^tm3Sado
Targeted Allele Detail

Summary

• Symbol: Tsix/Xist^tm3Sado
• Name: inactive X specific transcripts; targeted mutation 3, Takashi Sado
• MGI ID: MGI:3772875
• Synonyms: Tsix^pA
• Gene: Xist Location: ChrX:102503979-102526839 bp, - strand Genetic Position: ChrX, 46.15 cM, cytoband D
• Alliance: Tsix/Xist^tm3Sado page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130425
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A multiple polyadenylation sequence flanked by splice donor and acceptor sites was inserted into exon 4 of Tsix in its orientation. The insertion site is also within exon 1 of Xist on the other strand. The absence of transcript expression from Xist and Tsix was confirmed by RT-PCR. (J:130425)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Xist Mutation: 8 strains or lines available

Notes

Chimeric mice were bred with Tsix^tm1Enl mice to produce null male mice.

References

• Original: J:130425 Ohhata T, et al., Crucial role of antisense transcription across the Xist promoter in Tsix-mediated Xist chromatin modification. Development. 2008 Jan;135(2):227-35
• All: 2 reference(s)