Robo1^tm1Wian
Targeted Allele Detail

Summary

• Symbol: Robo1^tm1Wian
• Name: roundabout guidance receptor 1; targeted mutation 1, William Andrews
• MGI ID: MGI:3772898
• Synonyms: Dulox Robo^-, Robo1^-
• Gene: Robo1 Location: Chr16:72105194-72842983 bp, + strand Genetic Position: Chr16, 41.08 cM
• Alliance: Robo1^tm1Wian page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130430
• Parent Cell Line: CCB (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: In a two step integration process a hyg cassette with a 5' loxP site was inserted upstream of exon 1 and a puromycin resistance cassette with a 3' loxP site was inserted upstream of exon 22. Cre-mediated recombination was used to delete exons 1 through 21. The absence of protein expression was confirmed by immunohistochemistry. (J:130430)

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Robo1 Mutation: 87 strains or lines available

References

• Original: J:130430 Andrews W, et al., The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons. Dev Biol. 2008 Jan 15;313(2):648-58
• All: 12 reference(s)