Del(7Ins2-Tel)1Lef
Targeted Allele Detail

Summary

• Symbol: Del(7Ins2-Tel)1Lef
• Name: deletion, Chr 7, Louis Lefebvre 1
• MGI ID: MGI:3772925
• Synonyms: Del1Nagy, DelTel7
• Gene: Del(7Ins2-Tel)1Lef Location: unknown Genetic Position: Chr7, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130399
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Intergenic deletion, Intragenic deletion
• Mutation details: A neomycin resistance gene under the control of a floxed PGK promoter was placed 2.6 kb upstream of the Ins2 gene. After selection, the PGK promoter was removed by transient Cre expression, leaving a single loxP site upstream of the neomycin resistance gene. Chromosomal sequence distal of the Ins2 gene was then deleted by recombinase mediated chromosome truncation. This was done by using a targeting vector that had telomere repeats followed by a GFP expression cassette and a PGK promoter-loxP cassette suitable to activate the silent neo marker. Cre mediated recombination replaced the portion of chromosome 7 distal to the loxP-neo cassette with the targeting vector. ES cells with the intended deletion were selected by southern blotting and FISH analysis. Germline transmission was established through one chimeric male and subsequent genotyping performed by genomic PCR. (J:130399)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(7Ins2-Tel)1Lef Mutation: 0 strains or lines available

References

• Original: J:130399 Oh R, et al., Epigenetic and phenotypic consequences of a truncation disrupting the imprinted domain on distal mouse chromosome 7. Mol Cell Biol. 2008 Feb;28(3):1092-103
• All: 3 reference(s)