Kcnq1ot1^tm2Tilg
Targeted Allele Detail

Summary

• Symbol: Kcnq1ot1^tm2Tilg
• Name: KCNQ1 overlapping transcript 1; targeted mutation 2, Shirley M Tilghman
• MGI ID: MGI:3773025
• Synonyms: Promdelta
• Gene: Kcnq1ot1 Location: Chr7:142766848-142850284 bp, - strand Genetic Position: Chr7, 88.15 cM
• Alliance: Kcnq1ot1^tm2Tilg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108700
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: 244 bp of the gene promoter was deleted and a self-excising selection cassette was placed downstream of the gene. The deleted sequence included two of the three DNAse hypersensitive sites in the promoter and the gene transcription start site but left most of the CpG island intact. After recombination and generation of chimeric mice, the selection cassette excised itself in the male germline leaving behind a single loxP site. Heterozygous mice that inherit the deletion paternally do not express the gene transcript as determined by RNAse protection assay. (J:108700)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnq1ot1 Mutation: 0 strains or lines available

References

• Original: J:108700 Mancini-Dinardo D, et al., Elongation of the Kcnq1ot1 transcript is required for genomic imprinting of neighboring genes. Genes Dev. 2006 May 15;20(10):1268-82
• All: 3 reference(s)