Kcnq1ot1^tm3Tilg
Targeted Allele Detail

Summary

• Symbol: Kcnq1ot1^tm3Tilg
• Name: KCNQ1 overlapping transcript 1; targeted mutation 3, Shirley M Tilghman
• MGI ID: MGI:3773032
• Synonyms: K-term, Term
• Gene: Kcnq1ot1 Location: Chr7:142766848-142850284 bp, - strand Genetic Position: Chr7, 88.15 cM
• Alliance: Kcnq1ot1^tm3Tilg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108700
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A polyA based transcriptional stop element was inserted 1.5-kb downstream of the transcription start and a self-excising selection cassette was placed downstream of the gene. After recombination and generation of chimeric mice, the selection cassette excised itself in the male germline leaving behind the stop element and a single loxP site. Heterozygous mice that inherit the deletion paternally transcribe a truncated RNA that is missing the distal 58.5 kb of sequence. (J:108700)

Expression

In Mice Carrying this Mutation:

25 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnq1ot1 Mutation: 0 strains or lines available

References

• Original: J:108700 Mancini-Dinardo D, et al., Elongation of the Kcnq1ot1 transcript is required for genomic imprinting of neighboring genes. Genes Dev. 2006 May 15;20(10):1268-82
• All: 2 reference(s)