Tead1^tm1Hssk
Targeted Allele Detail

Summary

• Symbol: Tead1^tm1Hssk
• Name: TEA domain family member 1; targeted mutation 1, Hiroshi Sasaki
• MGI ID: MGI:3773642
• Gene: Tead1 Location: Chr7:112278563-112505991 bp, + strand Genetic Position: Chr7, 58.8 cM, cytoband F2
• Alliance: Tead1^tm1Hssk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131426
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A DNA fragment containing exon 2 and small segments of the flanking introns has been replaced by a loxP-flanked Pgk-neo-poly(A) cassette and a second cassette comprising an engrailed 2 splice acceptor (SA) followed by an internal ribosome entry site (IRES), the beta-galactosidase/neomycin fusion gene Betageo, and a polyadenylation signal. Reverse transcription- (RT-) PCR with primers for two downstream segments (exons 7-9 and 10-11) detects no transcripts in homozygous mutant embryos. (J:131426)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tead1 Mutation: 275 strains or lines available

References

• Original: J:131426 Sawada A, et al., Redundant roles of Tead1 and Tead2 in notochord development and the regulation of cell proliferation and survival. Mol Cell Biol. 2008 May;28(10):3177-89
• All: 2 reference(s)