Tead2^tm1Hssk
Targeted Allele Detail

Summary

• Symbol: Tead2^tm1Hssk
• Name: TEA domain family member 2; targeted mutation 1, Hiroshi Sasaki
• MGI ID: MGI:3773644
• Gene: Tead2 Location: Chr7:44865216-44882892 bp, + strand Genetic Position: Chr7, 29.19 cM, cytoband B5
• Alliance: Tead2^tm1Hssk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131426
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A DNA fragment encompassing the second and third exons and small segments of the first and third introns has been replaced by a cassette comprising an engrailed 2 splice acceptor (SA) followed by an internal ribosome entry site (IRES), the beta-galactosidase/neomycin fusion gene Betageo, and a polyadenylation signal. Although in homozygous mutant mice, no transcripts containing the deleted exons or exons 10-11 are detected by reverse transcription- (RT-) PCR, transcripts including exons 4-6 and 6-8 are detected; any translation products would be non-functional, lacking portions of the DNA binding and/or coactivator interaction domains. (J:131426)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tead2 Mutation: 34 strains or lines available

References

• Original: J:131426 Sawada A, et al., Redundant roles of Tead1 and Tead2 in notochord development and the regulation of cell proliferation and survival. Mol Cell Biol. 2008 May;28(10):3177-89
• All: 3 reference(s)