Snord116^tm1.1Uta
Targeted Allele Detail

Summary

• Symbol: Snord116^tm1.1Uta
• Name: small nucleolar RNA, C/D box 116 cluster; targeted mutation 1.1, Uta Francke
• MGI ID: MGI:3773673
• Synonyms: 1-lox, 1-loxp, Snord116del
• Gene: Snord116 Location: unknown Genetic Position: Chr7, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131427
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Snord116^tm1.1Uta involves 9 genes/genome features (Snord116, Snord116l2, Snord116l8 ...) View all
• Mutation details: Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with a cre expressing plasmid. The resulting 1-loxP ES cells (with the Snord116 cluster deleted and a single loxP site remaining) were injected into recipient blastocysts. (J:131427)

Disease models

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Snord116 Mutation: 3 strains or lines available

References

• Original: J:131427 Ding F, et al., SnoRNA Snord116 (Pwcr1/MBII-85) Deletion Causes Growth Deficiency and Hyperphagia in Mice. PLoS One. 2008;3(3):e1709
• All: 13 reference(s)