Pip5k1a^Gt(XE248)Byg
Gene trapped Allele Detail

Summary

• Symbol: Pip5k1a^Gt(XE248)Byg
• Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha; gene trap XE248, BayGenomics
• MGI ID: MGI:3773719
• Synonyms: PIP5KIbeta^-
• Gene: Pip5k1a Location: Chr3:94965841-95014241 bp, - strand Genetic Position: Chr3, 40.74 cM
• Alliance: Pip5k1a^Gt(XE248)Byg page

Mutation origin

• Mutant Cell Line: XE248
• Germline Transmission: Earliest citation of germline transmission: J:131289
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The beta-geo cassette was inserted into the first intron. RT-PCR and immunoblotting confirmed the absence of expression from this null allele. (J:131289)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE248	CC178278 ; (MGI Seq Detail)	5' RACE	Chr3:95013797-95013942 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  40 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pip5k1a Mutation: 67 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:131289 Wang Y, et al., Loss of PIP5KIgamma, unlike other PIP5KI isoforms, impairs the integrity of the membrane cytoskeleton in murine megakaryocytes. J Clin Invest. 2008 Feb;118(2):812-9
• All: 4 reference(s)