Pip5k1c^Gt(XD096)Byg
Gene trapped Allele Detail

Summary

• Symbol: Pip5k1c^Gt(XD096)Byg
• Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma; gene trap XD096, BayGenomics
• MGI ID: MGI:3773720
• Synonyms: PIP5KIgamma^-
• Gene: Pip5k1c Location: Chr10:81128797-81155807 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Pip5k1c^Gt(XD096)Byg page

Pip5k1c^Gt(XD096)Byg/Pip5k1c^Gt(XD096)Byg embryos are smaller and have open anterior neural folds

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: XD096
• Germline Transmission: Earliest citation of germline transmission: J:131432
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The beta-geo cassette was inserted into the first intron. The insertion leads to a read-through mutation within the first intron and truncates after the 32nd amino acid. RT-PCR indicates a complete loss of transcripts beyond the first exon. Embryos express beta-geo as indicated by lacZ staining. (J:131432)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XD096	CC178365 ; (MGI Seq Detail)	5' RACE	Chr10:81128898-81128987 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  5 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	10 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pip5k1c Mutation: 41 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:131432 Wang Y, et al., PIP5KI gamma is required for cardiovascular and neuronal development. Proc Natl Acad Sci U S A. 2007 Jul 10;104(28):11748-53
• All: 4 reference(s)