Spi1^tm3Dgt
Targeted Allele Detail

Summary

• Symbol: Spi1^tm3Dgt
• Name: Spi-1 proto-oncogene; targeted mutation 3, Daniel G Tenen
• MGI ID: MGI:3773989
• Synonyms: mRunx1^ki, PU.1-URE-mRunx
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm3Dgt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131217
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: The endogenous gene was replaced with one in which the three Runx1 binding sites within a floxed upstream regulatory element (URE) were mutated from TGTGGT to TGACCT by sire-directed mutagenesis. A frt-flanked neo cassette was inserted upstream of the URE. (J:131217)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 28 strains or lines available

References

• Original: J:131217 Huang G, et al., PU.1 is a major downstream target of AML1 (RUNX1) in adult mouse hematopoiesis. Nat Genet. 2008 Jan;40(1):51-60
• All: 2 reference(s)