Map1lc3b^tm1Mrab
Targeted Allele Detail

Summary

• Symbol: Map1lc3b^tm1Mrab
• Name: microtubule-associated protein 1 light chain 3 beta; targeted mutation 1, Marlene Rabinovtich
• MGI ID: MGI:3774111
• Synonyms: LC3B^-
• Gene: Map1lc3b Location: Chr8:122317177-122325499 bp, + strand Genetic Position: Chr8, 70.67 cM
• Alliance: Map1lc3b^tm1Mrab page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130322
• Parent Cell Line: CGR8 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The second amino acid encoded by exon 3 was replaced with a stop translation codon and the remainder of exon 3 and exon 4 were replaced with an IRES Tau-LacZ and neo cassette. The absence of endogenous protein product was confirmed on E14.5 head and trunk extracts and E18.5 head extracts. Beta-galactosidase is not expressed from this allele. (J:130322)

Expression

In Mice Carrying this Mutation:	9 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Map1lc3b Mutation: 27 strains or lines available

Notes

ES cell line = CGR8.8 (subline of CGR8)

References

• Original: J:130322 Cann GM, et al., Developmental expression of LC3alpha and beta: absence of fibronectin or autophagy phenotype in LC3beta knockout mice. Dev Dyn. 2008 Jan;237(1):187-95
• All: 24 reference(s)