Polr1b^{Gt(Ayu8019)Imeg}
Gene trapped Allele Detail

Summary

• Symbol: Polr1b^{Gt(Ayu8019)Imeg}
• Name: polymerase (RNA) I polypeptide B; gene trap Ayu8019, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:3774130
• Synonyms: Rpo1-2^Gt
• Gene: Polr1b Location: Chr2:128942915-128968514 bp, + strand Genetic Position: Chr2, 62.68 cM, cytoband F3
• Alliance: Polr1b^{Gt(Ayu8019)Imeg} page

Mutation origin

• Mutant Cell Line: Ayu8019
• Germline Transmission: Earliest citation of germline transmission: J:130226
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The gene trap vector pU-Hachi (containing En-2 intron, splice acceptor and a floxed IRES-beta-geo cassette) was inserted 202 bp downstream of exon 14. The presence of the lacZ transcript was confirmed by northern blot analysis on brain, heart, kung, kindey and testis extracts. (J:130226)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polr1b Mutation: 59 strains or lines available

References

• Original: J:130226 Chen H, et al., Early pre-implantation lethality in mice carrying truncated mutation in the RNA polymerase 1-2 gene. Biochem Biophys Res Commun. 2008 Jan 25;365(4):636-42
• All: 2 reference(s)