Mbnl2^{Gt(RRK149)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Mbnl2^{Gt(RRK149)Byg}
• Name: muscleblind like splicing factor 2; gene trap RRK149, BayGenomics
• MGI ID: MGI:3774137
• Synonyms: Mbnl2^GT2
• Gene: Mbnl2 Location: Chr14:120513081-120669109 bp, + strand Genetic Position: Chr14, 64.44 cM
• Alliance: Mbnl2^{Gt(RRK149)Byg} page

Mutation origin

• Mutant Cell Line: RRK149
• Germline Transmission: Earliest citation of germline transmission: J:130160
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: A gene trap containing a splice acceptor and a beta-geo cassette was inserted into intron 2. The absence of transcript was confirmed by northern blot analysis of skeletal muscle extract. (J:130160)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRK149	CC894141 ; (MGI Seq Detail)	5' RACE	Chr14:120562479-120562821 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  45 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mbnl2 Mutation: 66 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:130160 Hao M, et al., Muscleblind-like 2 (Mbnl2) -deficient mice as a model for myotonic dystrophy. Dev Dyn. 2008 Feb;237(2):403-10
• All: 3 reference(s)