Igh-8^{tm2(Ighg1)Aak}
Targeted Allele Detail

Summary

• Symbol: Igh-8^{tm2(Ighg1)Aak}
• Name: immunoglobulin heavy chain 8 (heavy chain of IgG3); targeted mutation 2, Ahmed Amine Khamlichi
• MGI ID: MGI:3774279
• Synonyms: Igamma1/Igamma3
• Gene: Igh-8 Location: Chr12:113329416-113330847 bp Genetic Position: Chr12, Syntenic
• Alliance: Igh-8^{tm2(Ighg1)Aak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130574
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The Igh-8 gene segment contains an upstream Igamma3 noncoding exon implicated in class switch recombination. A 2 kb PmeI-EcoRV fragment containing the Igamma3 promoter and the proximal part of the exon was replaced with 500 bp of the Igamma1 enhancer/promoter region of Ighg1. The remaining 368 bp portion of the Igamma3 exon contains the canonical splice donator site necessary for class switch recombination. A loxP site flanked neomycin resistance gene cassette was inserted upstream of the targeted region. The neo cassette was removed through subsequent cre-mediated recombination Germline transcripts containing the shortened sequence of the targeted Igamma 3 exon were detected by RT-PCR of RNA from activated splenocytes of homozygous mice. (J:130574)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh-8 Mutation: 5 strains or lines available

References

• Original: J:130574 Oruc Z, et al., Replacement of Igamma3 germ-line promoter by Igamma1 inhibits class-switch recombination to IgG3. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20484-9
• All: 1 reference(s)