Eif2ak4^tm1.1Dron
Targeted Allele Detail

Summary

• Symbol: Eif2ak4^tm1.1Dron
• Name: eukaryotic translation initiation factor 2 alpha kinase 4; targeted mutation 1.1, David Ron
• MGI ID: MGI:3774318
• Synonyms: Eif2ak4^tm1.1Munn, GCN2^cko, GCN2^conditional
• Gene: Eif2ak4 Location: Chr2:118219099-118305715 bp, + strand Genetic Position: Chr2, 59.4 cM, cytoband E5
• Alliance: Eif2ak4^tm1.1Dron page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:129841
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exon 12 was flanked by loxP sites by inserting a loxP sequence into the EcorRI site in intron 11, and a floxed neomycin resistance cassette into the NheI site of intron 12. After homologous recombination, Cre was transiently expressed and ES cells were selected that had the neomycin cassette removed. Geneotypes were confirmed by PCR. The floxed exon 12 encodes a region of the kinase domain required for ATP binding. In addition, removal of this exon leads to a frameshift mutation with multiple stop codons. (J:129841)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Eif2ak4 Mutation: 89 strains or lines available

References

• Original: J:129841 Maurin AC, et al., The GCN2 kinase biases feeding behavior to maintain amino acid homeostasis in omnivores. Cell Metab. 2005 Apr;1(4):273-7
• All: 12 reference(s)