Otx2^tm3Tlam
Targeted Allele Detail

Summary

• Symbol: Otx2^tm3Tlam
• Name: orthodenticle homeobox 2; targeted mutation 3, Thomas Lamonerie
• MGI ID: MGI:3774322
• Synonyms: Otx2^OG, Otx2^Otx2-GFP
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm3Tlam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130649
• Parent Cell Line: ENS (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: A cassette containing a GFP and frt-flanked neo cassette was inserted after the last coding region. The neo cassette was removed by flp-mediated recombination. Expression of the GFP fusion was confirmed by western blot analysis on E9.5 tail and head extracts. Authors state that this allele is hypomorphic. (J:130649)

Expression

In Mice Carrying this Mutation:	69 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:130649 Fossat N, et al., A new GFP-tagged line reveals unexpected Otx2 protein localization in retinal photoreceptors. BMC Dev Biol. 2007;7:122
• All: 7 reference(s)