Mylpf^tm1Jdp
Targeted Allele Detail

Summary

• Symbol: Mylpf^tm1Jdp
• Name: myosin light chain, phosphorylatable, fast skeletal muscle; targeted mutation 1, James D Potter
• MGI ID: MGI:3774323
• Synonyms: RLC^-
• Gene: Mylpf Location: Chr7:126808071-126813459 bp, + strand Genetic Position: Chr7, 69.39 cM
• Alliance: Mylpf^tm1Jdp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131461
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with a floxed neo cassette and an exon 2 containing a nucleotide substitution that resulted in an amino acid substitution of an alanine for an aspartic acid at position 49 (D49A). The absence of protein product was confirmed by western blot analysis on hindlimb extracts. This allele is a null allele. (J:131461)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mylpf Mutation: 27 strains or lines available

References

• Original: J:131461 Wang Y, et al., Fast skeletal muscle regulatory light chain is required for fast and slow skeletal muscle development. FASEB J. 2007 Jul;21(9):2205-14
• All: 1 reference(s)