Tcf3^tm5Zhu
Targeted Allele Detail

Summary

• Symbol: Tcf3^tm5Zhu
• Name: transcription factor 3; targeted mutation 5, Yuan Zhuang
• MGI ID: MGI:3774507
• Synonyms: E2A^GFP
• Gene: Tcf3 Location: Chr10:80245348-80269481 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Tcf3^tm5Zhu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75746
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The construct contained the Tcf3 gene in which the stop codon had been converted into a BamH1 restriction site by site-directed mutagenesis. The entire coding region of enhanced green fluorescent protein (EGFP) inserted in-frame into this BamH1 site. A PGKneo positive selection cassette was inserted downstream of the Tcf3 gene, and a PGK-driven Diphtheria Toxin gene was inserted outside the homologous regions for as a negative selection marker. Mice express functional Tcf3 as well as EGFP in hematopoietic stem cells, B lineage cells and erythroid lineage cells in bone marrow, as well as in peripheral B1 and B2 lineage B cells. (J:88032)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tcf3 Mutation: 43 strains or lines available

References

• Original: J:75746 Pan L, et al., An Analysis of T Cell Intrinsic Roles of E2A by Conditional Gene Disruption in the Thymus. J Immunol. 2002 Apr 15;168(8):3923-32
• All: 5 reference(s)