Clnk^tm1Rygo
Targeted Allele Detail

Summary

• Symbol: Clnk^tm1Rygo
• Name: cytokine-dependent hematopoietic cell linker; targeted mutation 1, Ryo Goitsuka
• MGI ID: MGI:3774508
• Synonyms: MIST^deltaneo
• Gene: Clnk Location: Chr5:38863805-39034155 bp, - strand Genetic Position: Chr5, 20.81 cM
• Alliance: Clnk^tm1Rygo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131738
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2, containing the start methionine, was replaced with an EGFP and floxed neo cassette. The neo cassette was removed by transient cre-mediated recombination. The absence of protein product was confirmed by western blot analysis on spleen NK and NKT cell extracts and mast cell extracts. (J:131738)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Clnk Mutation: 31 strains or lines available

References

• Original: J:131738 Sasanuma H, et al., Dual function for the adaptor MIST in IFN-gamma production by NK and CD4+NKT cells regulated by the Src kinase Fgr. Blood. 2006 May 1;107(9):3647-55
• All: 2 reference(s)