Notch1^tm2Pst
Targeted Allele Detail

Summary

• Symbol: Notch1^tm2Pst
• Name: notch 1; targeted mutation 2, Pamela Stanley
• MGI ID: MGI:3774549
• Synonyms: Notch1^12f
• Gene: Notch1 Location: Chr2:26347914-26393834 bp, - strand Genetic Position: Chr2, 18.91 cM
• Alliance: Notch1^tm2Pst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131563
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A floxed neo cassette in intron 5 and an exon 8 containing nucleotide substitutions that result in an amino acid substitution of alanine for threonine at position 466 (T466A). An additional LoxP site was inserted into intron 8. The neo cassette was removed by germ-line cre-mediated recombination. (J:131563)

Expression

In Mice Carrying this Mutation:	74 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch1 Mutation: 117 strains or lines available

References

• Original: J:131563 Ge C, et al., The O-fucose glycan in the ligand-binding domain of Notch1 regulates embryogenesis and T cell development. Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1539-44
• All: 9 reference(s)