Syt11^tm1Sud
Targeted Allele Detail

Summary

• Symbol: Syt11^tm1Sud
• Name: synaptotagmin XI; targeted mutation 1, Thomas C Sudhof
• MGI ID: MGI:3774591
• Gene: Syt11 Location: Chr3:88652006-88682466 bp, - strand Genetic Position: Chr3, 38.96 cM
• Alliance: Syt11^tm1Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131804
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to place a LoxP-Flp-neomycin-Flp cassette in intron 1, a FLAG-(C)4-FLAG cassette in exon 2, and LoxP site in intron 2. Mutant protein expression levels are similar to those of the wildtype protein. Expression is brain-specific and localized in the neuronal soma and dendrites (presumably on cargo vesicles). Protein can be recognized using FLAG antibody by Western blot analysis, but not in brain sections and labeled cultures. No significant N-ethyl-maleimide-sensitive factor attachment protein receptor (SNARE) binding is observed in immunoprecipitation (IP) assays. (J:131804)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Syt11 Mutation: 41 strains or lines available

References

• Original: J:131804 Sudhof TC, Generation of Syt11 mutant mice. MGI Direct Data Submission. 2008
• All: 5 reference(s)