Ptprj^tm1.2Weis
Targeted Allele Detail

Summary

• Symbol: Ptprj^tm1.2Weis
• Name: protein tyrosine phosphatase receptor type J; targeted mutation 1.2, Arthur Weiss
• MGI ID: MGI:3774766
• Synonyms: CD148^-, Ptprj^TM-
• Gene: Ptprj Location: Chr2:90260098-90410939 bp, - strand Genetic Position: Chr2, 50.19 cM, cytoband E1-2
• Alliance: Ptprj^tm1.2Weis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131736
• Parent Cell Line: E14.1TG3B1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was designed to introduce a loxP-flanked neomycin-resistance cassette in reverse transcriptional orientation to intron 17 and an additional loxP site to intron 18. Exon 18 encodes the transmembrane domain of the gene (also called CD148). Targeted animals were bred with BALB/c-background CMV-Cre transgenic female mice to delete the neomycin cassette and exon 18. Transmembrane domain-deleted mice were obtained and characterized. CD148 protein is not detected on any cells of hematopoietic origin by immunoblotting or flow cytometry. (J:131736)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ptprj Mutation: 71 strains or lines available

References

• Original: J:131736 Zhu JW, et al., Structurally distinct phosphatases CD45 and CD148 both regulate B cell and macrophage immunoreceptor signaling. Immunity. 2008 Feb;28(2):183-96
• All: 7 reference(s)