Ldhc^tm1Erg
Targeted Allele Detail

Summary

• Symbol: Ldhc^tm1Erg
• Name: lactate dehydrogenase C; targeted mutation 1, Erwin Goldberg
• MGI ID: MGI:3775042
• Synonyms: testis Ldh knockout
• Gene: Ldhc Location: Chr7:46510687-46527571 bp, + strand Genetic Position: Chr7, 30.6 cM
• Alliance: Ldhc^tm1Erg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132072
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was placed upstream of exon 3 replacing a 31 bp fragment and a floxed neo cassette was placed downstream of exon 3 replacing a 71 bp fragment. In vivo Cre mediated recombination removed the entire floxed region including exon 3. Western blot and immunohistochemical analysis confirmed the absence of protein expression in testes. (J:132072)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ldhc Mutation: 14 strains or lines available

References

• Original: J:132072 Odet F, et al., Expression of the gene for Lactate Dehydrogenase C: Ldhc is required for male fertility. Biol Reprod. 2008;79(1):26-34
• All: 5 reference(s)