Espl1^tm2Pzg
Targeted Allele Detail

Summary

• Symbol: Espl1^tm2Pzg
• Name: extra spindle pole bodies 1, separase; targeted mutation 2, Pumin Zhang
• MGI ID: MGI:3775062
• Synonyms: separase^{S1121A-flox-Puro}
• Gene: Espl1 Location: Chr15:102204701-102232792 bp, + strand Genetic Position: Chr15, 57.45 cM
• Alliance: Espl1^tm2Pzg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106531
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A floxed Puro cassette and a nucleotide substitution that resulted in an alanine for serine amino acid substitution at position 1121 (S1121A) were knocked into the open reading frame. The puromycin resistance cassette can be removed by germ line cre-mediated recombination to produce a conditional knock-in allele. (J:106531)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Espl1 Mutation: 95 strains or lines available

References

• Original: J:106531 Huang X, et al., Securin and separase phosphorylation act redundantly to maintain sister chromatid cohesion in mammalian cells. Mol Biol Cell. 2005 Oct;16(10):4725-32
• All: 4 reference(s)