Trp53^tm2Att
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2Att
• Name: transformation related protein 53; targeted mutation 2, Laura D Attardi
• MGI ID: MGI:3775090
• Synonyms: p53^LSL-VP16
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2Att page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131856
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 though 4 were replaced with a floxed stop cassette, which included 4 poly adenylation sites and a puromycin resistance cassette, and a VP16 cassette. Exons 2 through 4 encode the transactivating domain. The expected protein cannot be bound by Mdm2 and down-regulated by it. However, the presence of the STOP cassette prevents unrestrained expression of the chimeric protein. (J:131856)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:131856 Johnson TM, et al., Knockin mice expressing a chimeric p53 protein reveal mechanistic differences in how p53 triggers apoptosis and senescence. Proc Natl Acad Sci U S A. 2008 Jan 29;105(4):1215-20
• All: 1 reference(s)