Kpna1^{Gt(P017E08)Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Kpna1^{Gt(P017E08)Wrst}
• Name: karyopherin subunit alpha 1; gene trap P017E08, Wolfgang Wurst
• MGI ID: MGI:3775202
• Synonyms: Impalpha5^-
• Gene: Kpna1 Location: Chr16:35803693-35859479 bp, + strand Genetic Position: Chr16, 25.43 cM, cytoband B4-B5
• Alliance: Kpna1^{Gt(P017E08)Wrst} page

Mutation origin

• Mutant Cell Line: P017E08
• Germline Transmission: Earliest citation of germline transmission: J:131958
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: FlipROSAbetageo
• Mutation details: A gene trap vector (containing the betageo fusion gene) was inserted into the gene resulting in a null allele. The absence of protein product was confirmed by western blot analysis on testis, spleen, brain, lung and heart extracts. (J:131958)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  P017E08	CL301863 ; (MGI Seq Detail)	5' RACE	Chr16:35803701-35803877 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  22 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kpna1 Mutation: 68 strains or lines available

References

• Original: J:131958 Shmidt T, et al., Normal brain development in importin-alpha5 deficient-mice. Nat Cell Biol. 2007 Dec;9(12):1337-8; author reply 1339
• All: 7 reference(s)