Tcrb^tm1(Tcrb)Fwa
Targeted Allele Detail

Summary

• Symbol: Tcrb^tm1(Tcrb)Fwa
• Name: T cell receptor beta chain; targeted mutation 1, Frederick W Alt
• MGI ID: MGI:3775389
• Synonyms: Vbeta14^Rep
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:131995
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intergenic deletion
• Mutation details: ES cells containing the Tcrb^tm1Slek mutation that lacks the Dbeta2-Jbeta2 locus were again targeted to replace the Vbeta14 recombination signaling sequence (RSS) with germline Dbeta1 and Jbeta1.1 segments, their RSS, and intervening intronic sequence. A floxed neo resistance cassette used in the targeting was subsequently removed by transient Cre expression. The gene targeting also introduced a loxP site and an EcoRI site to distinguish between the targeted allele and the endogenous Vbeta 14 allele. Southern blots of thymus confirmed the presence of both germline and recombined versions of the knocked in gene segments. (J:131995)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 97 strains or lines available

References

• Original: J:131995 Ranganath S, et al., Productive coupling of accessible Vbeta14 segments and DJbeta complexes determines the frequency of Vbeta14 rearrangement. J Immunol. 2008 Feb 15;180(4):2339-46
• All: 1 reference(s)