Hfe^tm1.1Wsr
Targeted Allele Detail

Summary

• Symbol: Hfe^tm1.1Wsr
• Name: homeostatic iron regulator; targeted mutation 1.1, Wolfgang Stremmel
• MGI ID: MGI:3775648
• Synonyms: Hfe-KO
• Gene: Hfe Location: Chr13:23886017-23894837 bp, - strand Genetic Position: Chr13, 9.88 cM, cytoband A2-A4
• Alliance: Hfe^tm1.1Wsr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132141
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Mice carrying the mutant Hfe allele were crossed with a Cre deleter strain. Offspring mice retain the neomycin resistance cassette but lack exons 3-5 that encode for the alpha-2, the alpha-3 and transmembrane domains. RT-PCR revealed an abberant splace transcript that connects exon 2 to exon 6. Immunoblots on liver extracts demonstrated that no protein is produced from the mutant allele. (J:132141)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hfe Mutation: 35 strains or lines available

References

• Original: J:132141 Herrmann T, et al., Iron overload in adult Hfe-deficient mice independent of changes in the steady-state expression of the duodenal iron transporters DMT1 and Ireg1/ferroportin. J Mol Med. 2004 Jan;82(1):39-48
• All: 8 reference(s)