Igkrs^tm1Nemz
Targeted Allele Detail

Summary

• Symbol: Igkrs^tm1Nemz
• Name: immunoglobulin kappa chain recombining segment; targeted mutation 1, David Nemazee
• MGI ID: MGI:3775730
• Synonyms: RS^delta
• Gene: Igkrs Location: unknown Genetic Position: Chr6, Syntenic
• Alliance: Igkrs^tm1Nemz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132125
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The upstream recombination signal sequence (RSS) was replaced by a loxP flanked neomycin resistance gene. Founder mice were crossed with a transgenic mouse that expresses Cre in germline cells for the removal of the neomycin cassette. Genomic PCR and sequencing confirmed the 139 bp encompassing the RSS were replaced by 190 bp consisting of a loxP site flanked by vector sequence. (J:132125)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igkrs Mutation: 1 strain or line available

References

• Original: J:132125 Vela JL, et al., Rearrangement of mouse immunoglobulin kappa deleting element recombining sequence promotes immune tolerance and lambda B cell production. Immunity. 2008 Feb;28(2):161-70
• All: 2 reference(s)