Nras^tm1Tyj
Targeted Allele Detail

Summary

• Symbol: Nras^tm1Tyj
• Name: neuroblastoma ras oncogene; targeted mutation 1, Tyler Jacks
• MGI ID: MGI:3775824
• Synonyms: Nras^G12D, Nras^{Loxp-STOP-Loxp (LSL) G12D}, Nras^LSL-G12D
• Gene: Nras Location: Chr3:102965643-102975230 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Nras^tm1Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132357
• Parent Cell Line: V26.2 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site, a puromycin resistance gene cassette and a STOP cassette, and a second loxP site were inserted into intron 1 and a p.G12D activating mutation was introduced in exon 2. The allele will only be expressed after Cre-mediated deletion of the STOP cassette. (J:132357)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nras Mutation: 44 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Primary Melanoma of the CNS (J:198244) in mice homozygous for Nras^tm1Tyj and hemizygous for Tg(Tyr-cre)1Lru.

References

• Original: J:132357 Haigis KM, et al., Differential effects of oncogenic K-Ras and N-Ras on proliferation, differentiation and tumor progression in the colon. Nat Genet. 2008 Mar 30;40(5):600-8
• All: 44 reference(s)