Shh^tm6Amc
Targeted Allele Detail

Summary

• Symbol: Shh^tm6Amc
• Name: sonic hedgehog; targeted mutation 6, Andrew P McMahon
• MGI ID: MGI:3775846
• Synonyms: Shh::GFP
• Gene: Shh Location: Chr5:28661838-28672099 bp, - strand Genetic Position: Chr5, 14.39 cM
• Alliance: Shh^tm6Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132152
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A GFP cassette was inserted into exon 3 and an FRT site flanked neomycin resistance gene cassette was inserted between exon 2 and 3. The presence of the fusion protein and the absence of the endogenous product was confirmed by western blot analysis on E9.5 embryo extracts. This allele produces functional albeit inefficient protein product. (J:132152)

Expression

In Mice Carrying this Mutation:

5 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Shh Mutation: 48 strains or lines available

References

• Original: J:132152 Chamberlain CE, et al., Notochord-derived Shh concentrates in close association with the apically positioned basal body in neural target cells and forms a dynamic gradient during neural patterning. Development. 2008 Mar;135(6):1097-106
• All: 7 reference(s)