Ldlr^tm2(AFP)Alb
Targeted Allele Detail

Summary

• Symbol: Ldlr^tm2(AFP)Alb
• Name: low density lipoprotein receptor; targeted mutation 2, Arthur L Beaudet
• MGI ID: MGI:3775951
• Synonyms: LDLR-hAFP
• Gene: Ldlr Location: Chr9:21634872-21661215 bp, + strand Genetic Position: Chr9, 7.87 cM
• Alliance: Ldlr^tm2(AFP)Alb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132096
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Other
• Ldlr^tm2(AFP)Alb expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  AFP (174)

  Afp	(MGI:87951)

• Ldlr^tm2(AFP)Alb expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ldlr (MGI:96765)

• Mutation details: A cassette containing an IRES, human AFP and polyadenylation signal was inserted into the 3' untranslated region along with a floxed neo cassette and resulted in a duplication of exon 13 through part of exon 18. The neo cassette was removed by transient cre expression. Expression of AFP was confirmed by a quantitative immunoassay. (J:132096)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ldlr Mutation: 81 strains or lines available

Notes

Insertion of the expression cassette into the 3' untranslated region results in a duplication of exon 13 through part of exon 18.

References

• Original: J:132096 Li K, et al., A gene fusion method to screen for regulatory effects on gene expression: application to the LDL receptor. Hum Mol Genet. 2002 Dec 15;11(26):3257-65
• All: 1 reference(s)